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MAB1767

Sigma-Aldrich

Anti-Human MT1-MMP catalytic domain Antibody, clone 3G4.2

clone 3G4.2, Chemicon®, from mouse

Synonym(s):

MMP-14

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3G4.2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MMP14(4323)

General description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP′s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP-14 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.

Specificity

Clone 3G4.2 recognizes the catalytic domain of MT1-MMP, identifying the active form of the protein. MW is approximately 60 kDa.

Immunogen

Epitope: catalytic domain
Recombinant protein containing the catalytic domain of human MT1-MMP.

Application

Suggested dilutions:
Western blot: 1:1000 - 1:2000
Detect Human MT1-MMP catalytic domain using this Anti-Human MT1-MMP catalytic domain Antibody, clone 3G4.2 validated for use in WB, IC, IH.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs

Target description

60 kDa

Physical form

Format: Purified
Protein A purified immunoglobulin liquid in PBS, pH 7.2, containing 0.1% sodium azide.

Storage and Stability

1 year at 2-8°C from date of shipment

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7).
Golubkov, VS; Strongin, AY
The Journal of Biological Chemistry null
Samuel Sheehy et al.
Gene regulation and systems biology, 11, 1177625017713996-1177625017713996 (2017-06-22)
Signal-transducing functions driven by the cytoplasmic domain of membrane type-1 matrix metalloproteinase (MT1-MMP) are believed to regulate many inflammation-associated cancer cell functions including migration, proliferation, and survival. Aside from upregulation of the inflammation biomarker cyclooxygenase-2 (COX-2) expression, MT1-MMP's role in
Pameeka S Smith-Pearson et al.
The Journal of biological chemistry, 285(51), 40201-40211 (2010-10-13)
The Abl tyrosine kinases, Abl and Arg, play a role in the regulation of the actin cytoskeleton by modulating cell-cell adhesion and cell motility. Deregulation of both the actin cytoskeleton and Abl kinases have been implicated in cancers. Abl kinase
Sanghyun Hong et al.
Brain, behavior, and immunity, 60, 282-292 (2016-11-12)
Myelin basic protein (MBP) is an auto-antigen able to induce intractable pain from innocuous mechanical stimulation (mechanical allodynia). The mechanisms provoking this algesic MBP activity remain obscure. Our present study demonstrates that membrane type 1 matrix metalloproteinase (MT1-MMP/MMP-14) releases the
Tasuku Nishihara et al.
The Journal of biological chemistry, 290(6), 3693-3707 (2014-12-10)
Neuronal glial antigen 2 (NG2) is an integral membrane chondroitin sulfate proteoglycan expressed by vascular pericytes, macrophages (NG2-Mφ), and progenitor glia of the nervous system. Herein, we revealed that NG2 shedding and axonal growth, either independently or jointly, depended on

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