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Key Documents

ABS436

Sigma-Aldrich

Anti-ROCK2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Rho-associated coiled-coil containing protein kinase 2, Rho-associated protein kinase 2, Rho-associated coiled-coil-containing protein kinase II, p164 ROCK-2, ROCK2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

General description

Rho-associated protein kinase 2 (ROCK2), also known as Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase II, and p164 ROCK-2, is a member of the AGC serine/threonine protein kinase family. ROCK2 is a key regulator of actin cytoskeleton and cell polarity. It is involved in regulation of actin cytoskeleton organization, smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, stress fiber and focal adhesion formation, neurite retraction, motility and cell adhesion. Many of the key roles of ROCK2 are attributed to its ability to phosphorylate ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A, VIM, SORL1 and IRF4. ROCK2 regulates positively the activation of p42/MAPK1-p44/MAPK3 and the regulation of p90RSK/RPS6KA1 during myogenic differentiation. Alternatively, ROCK2 is a negative regulator of VEGF-induced angiogenic endothelial cell activation. Recent studies have shown that ROCK2 inhibits keratinocyte terminal differentiation and may play an important role in the timely initiation of centrosome duplication. It also may regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Additional evidence suggests that ROCK2 plays a critical role in the regulation of spine and synaptic properties in the hippocampus.

Immunogen

KLH-conjugated linear peptide corresponding to human ROCK2.

Application

Anti-ROCK2 Antibody is a highly specific rabbit polyclonal antibody, that targets Rho-associated protein kinase 2 & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ROCK2 in mouse brain tissue.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ROCK2 in 15 µg of mouse brain tissue lysate.

Target description

~160 kDa observed. Uncharacterized bands may appear in some lysate(s).

Linkage

Replaces: MABN1037

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The microenvironment shapes cell behavior and determines metastatic outcomes of tumors. We addressed how microenvironmental cues control tumor cell invasion in pediatric medulloblastoma (MB). We show that bFGF promotes MB tumor cell invasion through FGF receptor (FGFR) in vitro and that

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