AB6002
Anti-MMP-1 Antibody, hinge region
from rabbit, purified by affinity chromatography
Synonym(s):
Fibroblast collagenase, Matrix metalloproteinase-1, matrix metallopeptidase 1, interstitial collagenase, matrix metalloprotease 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MMP1(4312)
General description
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). MMP1, also known as interstitial collagenase, is the only enzyme able to initiate the breakdown of the interstitial collagens, types I, II, and III.
Specificity
Additional sequence homology is noted with mouse (81%) and rat (75%).
The antibody recognizes an internal domain of human MMP-1 containing the hinge region. It does not cross react with MMP-2, MMP-9, or MMP-13.
Immunogen
KLH-conjugated synthetic peptide corresponding to the hinge region of human MMP-1.
Application
Anti-MMP-1 Antibody, hinge region is an antibody against MMP-1 for use in WB.
Quality
Evaluated on a representative lot by Western blot on A431 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MMP-1 on 10 µg of A431 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MMP-1 on 10 µg of A431 cell lysate.
Target description
54 kDa In Western Blot, bands around 53 kDa and 51 kDa (proenzyme) are observed in reduced proteins.
Linkage
Replaces: 04-1122
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Katherine Bleavins et al.
Biological trace element research, 145(2), 257-267 (2011-09-02)
The purpose of this study was to assess insoluble salts containing gadolinium (Gd(3+)) for effects on human dermal fibroblasts. Responses to insoluble Gd(3+) salts were compared to responses seen with Gd(3+) solubilized with organic chelators, as in the Gd(3+)-based contrast
Seniz Inanc et al.
BioTechniques, 63(4), 174-180 (2017-10-20)
Collagen zymography is an SDS-PAGE-based method for detecting both the proenzyme and active forms of collagenases. Although collagen zymography is used for assessment of the matrix metalloproteinases MMP-1 and MMP-13, it can be difficult to detect these collagenases due to
Ling Zhang et al.
Scientific reports, 8(1), 10041-10041 (2018-07-04)
Lysine-specific demethylase 1 (LSD1) exerts dual effects on histone H3, promoting transcriptional repression via Lys4 (H3K4) demethylation or transcriptional activation through Lys9 (H3K9) demethylation. These activities are often exerted at transcriptional start sites (TSSs) and depend on the type of
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Compromised learning and memory is a common feature of multiple neurodegenerative disorders. A paradigm spatial memory impairment could be caused by developmental lead (Pb) exposure. Growing evidence implicates epigenetic modifications in the Pb-mediated memory deficits; however, how histone modifications exemplified
Characterisation of genome-wide PLZF/RARA target genes.
Spicuglia, S; Vincent-Fabert, C; Benoukraf, T; Tiberi, G; Saurin, AJ; Zacarias-Cabeza et al.
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