LC/MS Analysis of Polar Amino Acids on Ascentis^® Si (HILIC Mode)

column

Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] water; [C] acetonitrile

gradient

5% A constant; 10 to 16% B, 85 to 79% C in 20 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

0.1 g/L each in mobile phase