HPLC Analysis of Pain Relievers on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 μm particles (50536-U)

mobile phase

[A] water with 3% acetic acid; [B] methanol with 3% acetic acid; (79:21, A:B)

flow rate

2.0 mL/min

pressure

3290 psi (227 bar)

column temp.

45 °C

detector

UV,275 nm

injection

10 μL

sample

100 - 360 μg/mLin mobile phase