HPLC Analysis of Ginsenoside Standards on Ascentis^® Express C18

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A]: water; [B]: acetonitrile

gradient

held at 25% for 1.5 min; 25 to 85% B in 12 min; held at 85% B for 1 min

flow rate

1.0 mL/min

pressure

3481 psi (240 bar)

column temp.

60 °C

detector

UV, 205 nm

injection

25 μL

sample

50 μg/mL in water:methanol (82:18)