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HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18

HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18 application for SPE, application for HPLC

Materiales

Analytical column

Referencia del producto
Descripción
Precios

Columna para HPLC Discovery® HS F5

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Related product

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Descripción
Precios

Tubo de SPE Discovery® DSC-MCAX

bed wt. 100 mg, volume 3 mL, pk of 54

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

human urine spiked amphetamine and methylamphetamine at with 2 mg/mL

SPE tube/cartridge

Discovery DSC-MCAX, 100 mg/3mL (52783-U)

condition

DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol; 1 mL DI water

sample addition

1 mL

washing

DSC-MCAX SPE tube: 1 mL 50 mM ammonium acetate, pH 6.0; 1 mL 1M acetic acid; 1mL methanol; C-18 SPE tube: 1 mL DI water; 1 mL 20% methanol

elution

DSC-MCAX SPE tube: 1 mL 5% ammonium hydroxide in methanol; C-18 SPE tube: 1 mL methanol

eluate post-treatment

evaporate to dryness with nitrogen at room temperature, reconstitute in mobile phase

column

Discovery HS F5, 15 cm x 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM ammonium acetate, pH 4.5: [B] methanol; (35:65, A:B)

flow rate

2 mL/min

column temp.

40 °C

detector

UV, 210 nm

injection

10 μL

Descripción

Application

Sample Pre-Treatment:
1 mL human urine was spiked with 2 mg/mL amphetamine and methylamphetamine. The spiked sample was diluted 1:1 with 1:1 with 50 mM ammonium acetate, pH 6.0.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany