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Merck

X2252

Sigma-Aldrich

Xanthine Oxidase microbial

lyophilized powder, ≥7 units/mg solid

Sinónimos:

Schardingerenzyme, XO, hypoxanthine oxidase, XOD, Xanthine:oxygen oxidoreductase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

microbial

Quality Level

form

lyophilized powder

specific activity

≥7 units/mg solid

mol wt

~160 kDa

technique(s)

inhibition assay: suitable

color

brown

optimum pH

7.5-8.0

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... xdhA(947116) , xdhB(947205) , xdhC(945148)

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General description

Research area: Cell Cycle

Xanthine Oxidase (XO), a versatile molybdoflavoprotein, is one of the forms of xanthine oxidoreductases (XORs). XO is characterized with two flavin molecules (FAD), two molybdenum atoms, and eight iron atoms bound per enzymatic unit.

Application

Xanthine Oxidase microbial has been used:
  • to determine in vitro xanthine oxidase activity
  • as a source to generate superoxide anions during superoxide dismutase assay
  • as a component of the reagent mix in colorimetric paper-based device to detect allopurinol in traditional medicine by enzymatic reaction

Biochem/physiol Actions

Xanthine Oxidase catalyzes the hydroxylation of hypoxanthine to xanthine and xanthine to uric acid and oxygen free radicals during purine metabolism. This enzyme is also implicated in caffeine metabolism. XO also plays a vital role in the catabolism of xenobiotics including various drugs such as thiopurine drugs, containing 6-mercaptopurine, allopurinol, and uric acid. Excess of XO activity causes hyperuricemia (higher levels of uric acid in the blood), which further leads to gout.
Also contains proteolytic activity.

Physical properties

Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Lyophilized powder containing BSA and sodium glutamate as stabilizers

Storage and Stability

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Pál Pacher et al.
Pharmacological reviews, 58(1), 87-114 (2006-03-02)
The prototypical xanthine oxidase (XO) inhibitor allopurinol, has been the cornerstone of the clinical management of gout and conditions associated with hyperuricemia for several decades. More recent data indicate that XO also plays an important role in various forms of
Michael C Hall
Molecular and cellular biochemistry, 292(1-2), 1-11 (2006-09-20)
The cytotoxicity of five oxysterols identified in cooked fish, 7-ketocholesterol, 7beta-hydroxycholesterol, cholesterol 5alpha,6alpha-epoxide, cholestanetriol and 4-cholesten-3-one, was investigated in primary cultures of bovine ovarian granulosa cells. Cells were exposed to the oxysterols individually and to a mixture of the same
P J Marro et al.
Pediatric research, 41(4 Pt 1), 513-520 (1997-04-01)
The effect of allopurinol to inhibit purine metabolism via the xanthine oxidase pathway in neonates with severe, progressive hypoxemia during rescue and reperfusion with extracorporeal membrane oxygenation (ECMO) was examined. Twenty-five term infants meeting ECMO criteria were randomized in a
Giuseppe Faudone et al.
Journal of medicinal chemistry, 64(11), 7156-7178 (2021-05-22)
The purine alkaloid caffeine is the most widely consumed psychostimulant drug in the world and has multiple beneficial pharmacological activities, for example, in neurodegenerative diseases. However, despite being an extensively studied bioactive natural product, the mechanistic understanding of caffeine's pharmacological
COLORIMETRIC PAPER-BASED DEVICE BY ENZYMATIC REACTION FOR DETECTING ALLOPURINOL IN TRADITIONAL MEDICINE
Rimadani P, et al.
Rasayan Journal of Chemistry (2021)

Protocolos

The appearance of uric acid is measured at 293nm by spectrophotometry.

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