U4883

Urea

8 M (after reconstitution with 16 mL high purity water)

• Sinónimos: Urea solution, Carbamide, Carbonyldiamide
• Fórmula lineal: NH₂CONH₂
• Número CAS: 57-13-6
• Peso molecular: 60.06
• UNSPSC Code: 12352111
• NACRES: NA.25
• PubChem Substance ID: 24900649
• EC Number: 200-315-5
• Beilstein/REAXYS Number: 635724
• MDL number: MFCD00008022

Propiedades

• form: powder
• concentration: 8 M (after reconstitution with 16 mL high purity water)
• solubility: H₂O: soluble (1 vial plus 16 mL)
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N

Descripción

General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. It can also disturb hydrogen bonding present in DNA secondary structure.

Application

Useful for solubilizing and denaturing proteins in preparation for 2D-PAGE, enzymatic digests, IMAC Chromatography, and related procedures.
Urea has been used for protein sample preparation for reverse-phase nanospray liquid chromatography-tandem mass spectrometry.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)