T8778

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

• Sinónimos: α,α-Trehalose glucohydrolase
• Número CAS: 9025-52-9
• Número CE: 3.2.1.28 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00132462
• Specific activity: ≥1.0 units/mg protein
• Concentration: 0.5-10.0 mg/mL protein (BCA)

Propiedades

• form: buffered aqueous glycerol solution
• Quality Segment: 200
• specific activity: ≥1.0 units/mg protein
• concentration: 0.5-10.0 mg/mL protein (BCA)
• storage temp.: −20°C

Descripción

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Application

Trehalase has been used:

• in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
• in a study to investigate growth arrest by trehalose-6-phosphate
• to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Physical form

Solution in 50% glycerol containing 1% Triton^™ X-100 and 25 mM potassium phosphate, pH 6.5

Other Notes

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable