T1428
apo-Transferrin bovine
BioReagent, suitable for cell culture, ≥98%
Sinónimos:
Human serotransferrin
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About This Item
Productos recomendados
biological source
bovine
Quality Level
product line
BioReagent
assay
≥98%
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
iron, essentially free
solubility
H2O: 10 mg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... TF(280705)
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General description
apo-Transferrin is an iron-free form of transferrin that is present in circulation.
Application
All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.
apo-Transferrin bovine has been used as a component of:
- basal medium for culturing bovine mammary epithelial cells
- minimum essential medium α (MEM-α) for spermatogonial stem cells (SSCs) culture
- induction medium for mesenchymal stem cell culture
Biochem/physiol Actions
apo-Transferrin (apoTf) aids in preventing iron accumulation in tissues, especially in non-transfusion dependent β-thalassemia.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
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Los clientes también vieron
Keita Abe et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 154-156 (2008-04-05)
We have investigated the mechanism of manganese ion uptake by apo-transferrin using a capillary electrophoresis method, and obtained clear evidence that oxidation state +3 and the binuclear unit of a manganese chelate are essential factors for the facile uptake by
Einar Vargas-Bello-Pérez et al.
Animal nutrition (Zhongguo xu mu shou yi xue hui), 5(2), 202-208 (2019-06-14)
The objective of this study was to determine how cytosolic triacylglycerols (TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids (FA). This objective was accomplished by addition of different FA to
Monique P Gelderman et al.
Haematologica, 100(5), 611-622 (2015-01-27)
Intermediate beta-thalassemia has a broad spectrum of sequelae and affected subjects may require occasional blood transfusions over their lifetime to correct anemia. Iron overload in intermediate beta-thalassemia results from a paradoxical intestinal absorption, iron release from macrophages and hepatocytes, and
Jia Wang et al.
Stem cell research & therapy, 10(1), 227-227 (2019-07-31)
High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still
Shanshan Li et al.
Journal of animal science and biotechnology, 7, 44-44 (2016-08-06)
The objective of this study was to study how changing the ratio of Lys to Thr, Lys to His, and Lys to Val affects the expression of lipogenic genes and microRNA (miRNA) in bovine mammary epithelial cells. Triplicate cultures with
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