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T0167

D-(+)- Trealosa dihydrate

from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sinónimos:

α,α-Trealosa, α-D-Glucopiranosil-α-D-glucopiranósido

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Fórmula empírica (notación de Hill):
C12H22O11 · 2H2O
Número CAS:
Peso molecular:
378.33
UNSPSC Code:
12352201
NACRES:
NA.75
PubChem Substance ID:
EC Number:
202-739-6
Beilstein/REAXYS Number:
5322018
MDL number:
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Quality Segment

biological source

Saccharomyces cerevisiae

product line

BioReagent

assay

≥99%

form

powder

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

solubility

H2O: 50 mg/mL

SMILES string

[H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1

InChI key

DPVHGFAJLZWDOC-PVXXTIHASA-N

General description

Trehalose is a non-reducing disaccharide, ubiquitously present in nature. It is used as a food preservative and also to retain enzymes, vaccines, cells and many other molecules. Trehalose is present as hemolymph sugar among the invertebrates.

Application

D-(+)-Trehalose dihydrate has been used in a slow-freezing method for freezing rabbit oocytes. It has also been used in solid-surface vitrification (SSV) of in vitro-matured oocytes. It is suitable for use as a cryoprotectant in a variety of cell freezing media.

Biochem/physiol Actions

Trehalose is essential for embryogenesis, carbon metabolism, photosynthesis and flowering in plants. It predominantly exists among the species that supports anhydrobiosis (life without water). It serves as a carbohydrate reserve in microorganisms and protects them from adverse conditions, such as heat, cold, dehydration, desiccation and oxidation. Trehalose acts as a chemical chaperone affecting protein folding. It is known to prevent amyloid generation and accummulation of β-amyloid in vitro. A similar effect of trehalose has been observed with huntingtin protein aggregation. Thus, it might serve as a protective agent in Alzheimer′s disease and Huntington′s disease.

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Este artículo
T9531T9449T5251
assay

≥99%

assay

≥99% (HPLC)

assay

≥99% (HPLC)

assay

≥98.5% (HPLC)

biological source

Saccharomyces cerevisiae

biological source

Saccharomyces cerevisiae

biological source

Manihot esculenta root (Cassava)

biological source

Saccharomyces cerevisiae

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

HPLC: suitable, PCR: suitable, cell based assay: suitable, cryopreservation: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable, cryopreservation: suitable

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

form

powder

form

powder

form

powder

form

powder

product line

BioReagent

product line

-

product line

-

product line

-


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–6 of 6 Questions  
  1. What is the average particle size in microns or particle mesh size for this product, please? This is important for my research work.

    1 answer
    1. The particle size of this product is not determined.

      Helpful?

  2. We would like to dissolve trehalose in water in bulk and store it. Does dissolved trehalose loose its stability after an extended storage time? Which storage temperature would you recommend?

    1 answer
    1. The solution stability and storage of this product has not been tested, however other commercial sources offer 1M Trehalose solutions that are stored at ambient temperature or refrigerated (filter-sterilized). Trehalose is added to several of less stable protein preparations as a carrier and/or cryoprotectant. An example would be EGFR, E2645. This lyophilized product contains 10% Trehalose. Upon reconstitution the product can be stored for up to 1 year at -70 deg C. Given the information above, Trehalose may be quite stable in solution.

      Helpful?

  3. Can solutions of Product T0167, D-(+)-Trehalose dihydrate, be autoclaved?

    1 answer
    1. Do not autoclave. It is best to sterile filter solutions.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Can Product T0167, D-(+)-Trehalose dihydrate, be used as a substitute for protein in a freezing media?

    1 answer
    1. Yes, it should work as a substitute for protein in a freezing media.  It is commonly used at a final concentration of 5% (50 mg/mL).  Alternatively, one could use methylcellulose.

      Helpful?

  6. Do we have information of any fluorescent impurities in Product T0167, D-(+)-Trehalose dihydrate?

    1 answer
    1. This product is not tested for the presence or absence of fluorescent impurities.

      Helpful?

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