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SEQXE

Sigma-Aldrich

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Sinónimos:

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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About This Item

Código UNSPSC:
41121800
NACRES:
NA.55
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

200

técnicas

whole genome amplification: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Categorías relacionadas

Descripción general

SeqPlex™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD™, or 454 sequencing workflows.

Aplicación

SeqPlex™ DNA Amplification Kit has been used for whole genome amplification.[1][2][3]

Características y beneficios

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD™, or 454 library prep for next generation sequencing

Otras notas

SEQXE-500RXN is manufactured on-demand. Contact technical services at [email protected] for more information.

Información legal

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS
  • Library Preparation Buffer

Opcional

Referencia del producto
Descripción
Precios

obligatorio pero no suministrado

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLCR4994
SLCP3644
SLCR4994
SLCQ9361
SLCR3884

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Visite la Librería de documentos

Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR).
Kunnev D, et al.
Journal of biological methods, 2(4) (2015)
Syuzo Kaneko et al.
Cancers, 13(9) (2021-05-01)
Although chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) using formalin-fixed paraffin-embedded tissue (FFPE) has been reported, it remained elusive whether they retained accurate transcription factor binding. Here, we developed a method to identify the binding sites of the insulator transcription factor
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Protocolos

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

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