SCA20

Sucrose Assay Kit

sufficient for 20 assays

• Sinónimos: Sucrose Test Kit
• UNSPSC Code: 12164500
• EC Number: 200-334-9
• NACRES: NA.84

Propiedades

• usage: sufficient for 20 assays
• Quality Segment: 100
• analyte chemical class(es): sugars (sucrose)
• technique(s): photometry: suitable
• application(s): food and beverages; general analytical
• storage temp.: 2-8°C

Descripción

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Solo componentes del kit

• glucose assay reagent 50 mL
• sucrose assay reagent 1 mL/vial
• sucrose standard 500 mg

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 3