SAE0097

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) from Acidaminococcus fermentans

recombinant, expressed in E. coli, aqueous solution

• Sinónimos: D2HGDH, HGDH, L-2-hydroxyglutarate dehydrogenase
• Número CE: 1.1. 99.2
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥1000 units/mg protein
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in E. coli

Propiedades

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥1000 units/mg protein
• UniProt accession no.: D2RJU7
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD⁺ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of α-ketoglutarate (α--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD⁺ .

The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:

• A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate.
• Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates.
• A coupled assay system to measure branched-chain amino acid aminotransferase activity.

Preparation Note

This recombinant D2HGDH product is supplied as an aqueous solution in 20 mM Trizma^® buffer, pH 7.5, with 150 mM NaCl, and 10% glycerol.

Other Notes

One unit of enzyme oxidizes 1 μmole of NADH to NAD⁺ coupled to the reduction of α-ketoglutarate to (D)-2-hydroxyglutarate per minute at 37°C at pH 8.0.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• ¿Qué está pasando?: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable