SAB4200749

Anti-Collagen Type III (COL3A1) Antibody

mouse monoclonal, FH-7A

• Sinónimos: Anti-COL3A1, Anti-Collagen alpha-1(III) chain
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• Nombre del producto: Anti-Collagen Type III antibody, Mouse monoclonal, clone FH-7A, purified from hybridoma cell culture
• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: FH-7A, monoclonal
• Formulario: buffered aqueous solution
• mol peso: ~70 kDa
• reactividad de especies: rat, human
• concentración: ~1.0 mg/mL
• técnicas: ELISA: suitable; immunoblotting: suitable; immunofluorescence: suitable; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat skin sections.
• isotipo: IgG1
• Nº de acceso UniProt: P02461
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... COL3A1(1281)

Descripción

Descripción general

Anti-collagen type III antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.
Monoclonal anti-collagen type III specifically recognizes native and denatured collagen type III from human 1 and rat 2 origin. It does not recognize collagen types I, II, IV, V, VI and X.
Collagen type III, also known as collagen α-1(III) (COL3A1), is encoded by the gene mapped to human chromosome 2q24.3-q31. It is the highly expressed collagen in blood vessels and hollow organs.

Inmunógeno

Human Collagen Type III

Acciones bioquímicas o fisiológicas

Collagen a-1(III) (COL3A1) serves as a potent adjunct marker for both differentiating fibroadenoma (FA) from phyllodes tumor (PT) and measuring malignant potential in PTs. Overexpression of the gene in glomerular leads to the development of collagen type III glomerulopathy. Mutations in the gene is associated with the development of familial aortic aneurysms and Ehlers-Danlos syndrome.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Otras notas

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable