SAB4200483

Anti-JAK2 antibody, Mouse monoclonal

clone 10.1.5, purified from hybridoma cell culture

• Sinónimos: Anti-JAK-2, Anti-JTK10, Anti-Janus kinase 2, Monoclonal Anti-JAK2 antibody produced in mouse
• Código UNSPSC: 12352203
• NACRES: NA.44

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: 10.1.5, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~125 kDa
• reactividad de especies: bovine, human
• concentración: ~1.0 mg/mL
• técnicas: indirect immunofluorescence: 2.5-5.0 μg/mL using MCF7 cells.; western blot: 2.0-4.0 μg/mL using TF1 total cell extracts.
• Nº de acceso UniProt: O60674
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... JAK2(3717)

Descripción

Descripción general

Monoclonal Anti-JAK2 (mouse IgG1 isotype) is derived from the hybridoma 10.1.5 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The Janus kinases (Jak) family of proteins includes four members: Jak1, Jak2, Jak3 and the tyrosine kinase 2 (Tyk2). Jak2 is ubiquitously expressed. It is also expressed in normal and neoplastic mammary epithelial cells.

Inmunógeno

synthetic peptide corresponding to an internal sequence of human JAK2 (GeneID 3717), conjugated to KLH.

Aplicación

Monoclonal Anti-JAK2 antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• flow cytometry

Acciones bioquímicas o fisiológicas

Janus kinases engage in interactions with the intracellular tails of several cytokine receptors, which result in the activation of members of the signal transducer and activator of transcription (STAT) family of transcription factors. The latter bind specific cognate sequences at the promoter region of genes that regulate proliferation and differentiation. JAK activation plays an important role in cell proliferation, differentiation, migration and apoptosis. A member of this family, JAK2, has been found to induce signaling regulating gene expression of various pro-survival and anti-apoptotic molecules. Furthermore, inhibition of JAK2 signaling triggered cell death in human cancer cells. These findings indicate JAK2 inhibition as a potential therapeutic approach for various cancers.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable