SAB4200095

Anti-PKM2 (isoform M2) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Sinónimos: Anti-CTHBP, Anti-OIP3(OPA-interacting protein 3), Anti-PK3, Anti-PKM, Anti-Pyruvate Kinase, MUSCLE (isoform M1), Anti-TCB, Anti-THBP1 (thyroid hormone-binding protein, cytosolic)
• Código UNSPSC: 12352203
• NACRES: NA.44

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~60 kDa
• reactividad de especies: human
• concentración: ~1.5 mg/mL
• técnicas: immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human colon carcinoma; immunoprecipitation (IP): 2-4 μg using HT29 cell lysates; indirect immunofluorescence: 4-8 μg/mL using HeLa cells; western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PKM2(5315)

Descripción

Descripción general

Pyruvate Kinase (PK) has four known isoforms, namely, L, R, M1 and M2. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. The M2 isoform has been linked to cancer metastasis .

Especificidad

Anti-PKM2 (isoform M2) antibody is specific for human PKM2 (isoform M2). The product is expected to react with rat and mouse PKM2. In immunoblotting, detection of the PKM2 (isoform M2) band is specifically inhibited by the PKM2 (isoform M2) immunizing peptide.

Aplicación

Anti-PKM2 (isoform M2) antibody produced in rabbit has been used in:

• chromatin immunoprecipitation (chip) assay
• western blotting
• immunofluorescence
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis. Tumor cells have been reported to exclusively overexpress the embryonic M2 isoform. The tumor metabolome is characterized by high glycolytic turnover rate and tumor cells are able to proliferate under conditions of aerobic glycolysis, known as the Warburg effect. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable