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S5394

Sigma-Aldrich

Lipoprotein Deficient Serum from fetal calf

frozen liquid

Sinónimos:

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.61
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

bovine fetus

Nivel de calidad

200

Formulario

frozen liquid

concentración

≥14 mg protein/mL Biuret

técnicas

cell culture | mammalian: suitable
cell culture | stem cell: suitable

impurezas

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum. [1]

Aplicación

Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells[2], thymic lobes[3] and human peripheral blood mononuclear cells (PBMCs).[4]

Nota de preparación

Aseptically filtered, 0.2 μm
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLCJ5148
SLBW0309
SLBQ5608V
SLBN4785V
SLBL5464V

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Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Liliana M R Silva et al.
Frontiers in cellular and infection microbiology, 12, 809606-809606 (2022-03-01)
Apicomplexan parasites are considered as defective in cholesterol synthesis. Consequently, they need to scavenge cholesterol from the host cell by either enhancing the uptake of extracellular cholesterol sources or by upregulating host cellular de-novo biosynthesis. Given that Eimeria bovis macromeront
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Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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