For use in Equine sources, Protein G has a significantly higher affinity for IgG, as compared to protein A. Please see the link below to review a comparison table of Protein A to Protein G by species.
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General description
Application
Biochem/physiol Actions
Packaging
Physical form
Preparation Note
Disclaimer
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| description lyophilized powder | description This Protein A, Peroxidase Conjugate is validated for use in Direct Immunoassay. | description lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein | description recombinant, expressed in unspecified host, lyophilized powder |
| technique(s) direct ELISA: 1:80,000 using human IgG | technique(s) - | technique(s) - | technique(s) direct ELISA: 1:100,000 using human IgG |
| form lyophilized powder | form liquid | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| conjugate peroxidase conjugate | conjugate - | conjugate - | conjugate peroxidase conjugate |
| Quality Level 200, 100 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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Hello, I would like to know if this conjugate serve for testing equine in a ELISA (Equus ferus caballus). Thank you
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I prepare a stock solution of Product P8651, Protein A Peroxidase?
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A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL.
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How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?
1 answer-
For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000.
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I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?
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P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.
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What is the solution stability of Product P8651, Protein A Peroxidase?
1 answer-
The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C.
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