P7999
Plasminogen from human plasma
≥2.0 units/mg protein, lumps and powder, suitable for streptokinase determination by clot formation procedure
Sinónimos:
Profibrinolysin
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
42030240
NACRES:
NA.54
Productos recomendados
origen biológico
human plasma
Formulario
lumps and powder
actividad específica
≥2.0 units/mg protein
mol peso
90 kDa
composición
protein, 2.0-7.0%
idoneidad
suitable for streptokinase determination by clot formation procedure
Nº de acceso UniProt
actividad extraña
plasmin ≤0.5% of base activity
temp. de almacenamiento
2-8°C
Información sobre el gen
human ... PLG(5340)
¿Está buscando productos similares? Visita Guía de comparación de productos
Descripción general
The plasminogen (PLG) gene is mapped to human chromosome 6q26. Plasminogen protein has a molecular weight of ?90kDa. It is composed of Pan-apple domain at the N-terminal, five kringle domains and serine protease domain at its C-terminal.
Aplicación
Plasminogen is a single-chain glycoprotein found in human plasma and extracellular fluid. Certain activators, such as tissue plasminogen activator (tPA), convert plasminogen to its active form, plasmin. Plasminogen has been used to study its conversion into plasmin.
The enzyme from Sigma has been used to examine the ability of plasminogen in nasal secretion to promote S. aureus surface colonization. It has been used to assess the binding of recombinant OspC protein to plasminogen using enzyme-linked immunosorbent assay. It has also been used to screen FITC-labeled S. pneumoniae for protein interaction by solid-phase assay. In this assay; a well was coated with plasminogen and incubated with FITC-labeled bacteria.The fluorescence signal was read in a fluorimeter.
Acciones bioquímicas o fisiológicas
Plasminogen is the inactive precursor of the protease plasmin. Plasminogen is activated by the action of either tissue plasminogen activator (tPA), which primarily activates the fibrinolytic (thrombolytic) activity of plasmin, or urokinase plasminogen activator (uPA), which is associated with extracellular matrix remodeling and cell migration. Plasmin cleaves fibrin/fibrinogen and blood coagulation factors V/Va and VIII/VIIIa. It activates matrix metalloproteinases by cleaving the inactive proenzymes. It is also involved in the activation of some growth factors, such as vascular endothelial growth factor (VEGF) and transforming growth factor β (TGF-β).
Propiedades físicas
Native-intact human plasminogen is a 791 amino acid glyco-protein with as many as 24 disulfide bonds. Plasminogen contains a single N-linked sialylated biantennary glycan. The two O-glycans possess a Gal β-1-3GalNAc core which are α-2-3 sialylated at the terminal Gal. An additional disialylated form has a second sialic acid residue with an α-2-6 linkage to GalNAc. Mono- and disialylated forms occur at a molar ratio of 80:20 in human plasminogen.
Definición de unidad
One unit will produce one micromole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C. Activity is determined after activation to plasmin with urokinase.
Reconstitución
Solutions should be stored in buffer containing 20 mM lysine at neutral pH. The protein should be as concentrated as possible. Frozen in aliquots at −20 °C, it should be stable for several weeks.
Nota de análisis
ε-Aminocaproic acid free
Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Christopher G Earnhart et al.
Clinical and vaccine immunology : CVI, 18(6), 901-906 (2011-04-29)
Borrelia burgdorferi OspC is an outer membrane lipoprotein required for the establishment of infection in mammals. Due to its universal distribution among B. burgdorferi sensu lato strains and high antigenicity, it is being explored for the development of a next-generation
Genetic risk factors for portopulmonary hypertension in patients with advanced liver disease
Roberts KE, et al.
American Journal of Respiratory and Critical Care Medicine, 179(9), 835-842 (2009)
Jian Sha et al.
Journal of bacteriology, 191(9), 3095-3107 (2009-03-10)
In this study, we demonstrated that the surface-expressed enolase from diarrheal isolate SSU of Aeromonas hydrophila bound to human plasminogen and facilitated the latter's tissue-type plasminogen activator-mediated activation to plasmin. The bacterial surface-bound plasmin was more resistant to the action
Yan Wang et al.
Journal of cellular and molecular medicine, 24(16), 9255-9266 (2020-07-07)
Blood-brain barrier (BBB) integrity injury within the thrombolytic time window is becoming a critical target to reduce haemorrhage transformation (HT). We have previously reported that BBB damage was initially damaged in non-infarcted striatum after acute ischaemia stroke. However, the underlying
Cécile Frolet et al.
BMC microbiology, 10, 190-190 (2010-07-14)
Streptococcus pneumoniae is a widely distributed commensal Gram-positive bacteria of the upper respiratory tract. Pneumococcal colonization can progress to invasive disease, and thus become lethal, reason why antibiotics and vaccines are designed to limit the dramatic effects of the bacteria
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico