P7640

Phosphatase, Alkaline from bovine intestinal mucosa

lyophilized powder, ≥10 DEA units/mg solid

• Sinónimos: Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Número CAS: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• EC Number: 232-631-4
• NACRES: NA.54
• MDL number: MFCD00131849
• Número CE: 3.1.3.1 (BRENDA, IUBMB)
• Specific activity: ≥10 DEA units/mg solid
• Biological source: bovine intestinal mucosa

Propiedades

• biological source: bovine intestinal mucosa
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥10 DEA units/mg solid
• mol wt: dimer ~160 kDa
• technique(s): protein detection: suitable
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P7640 has been used to study the monoclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex.

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)