P6746
Anti-PML antibody, Mouse monoclonal
clone PML-97, purified from hybridoma cell culture
Sinónimos:
Anti-Promyelocytic Leukemia
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About This Item
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
PML-97, monoclonal
Formulario
buffered aqueous solution
mol peso
antigen ~90 kDa
reactividad de especies
hamster, human, mouse
técnicas
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using 293T cells expressing recombinant PML3
isotipo
IgG1
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PML(5371)
Descripción general
Monoclonal Anti-PML (mouse IgG1 isotype) is derived from the PML-97 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with recombinant full-length human PML (promyelocytic leukemia) protein. PML is characterized with cysteine-rich zinc-binding domains (RING), two B-boxes, an adjacent leucine coiled coil forming the RBCC or tripartite motif (TRIM), and a nuclear localization signal (NLS). The RING and B-box are important for the interaction of the PML protein with different proteins. In normal cells, the PML protein is found in nuclear bodies (PML NBs) whereas in acute promyelocytic leukemia (APL) patients, the PML NBs are disrupted and delocalized from PML NBs to nucleus and cytoplasm.
The PML gene is located on the human chromosome at 15q24.1.
Especificidad
Monoclonal Anti-PML recognizes human, mouse, and hamster PML (about 90 kDa)
Inmunógeno
recombinant full-length human PML (promyelocytic leukemia) protein.
Aplicación
Anti-PML antibody, Mouse monoclonal may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- indirect immunofluorescence
Acciones bioquímicas o fisiológicas
A monoclonal antibody to promyelocytic leukemia (PML) is an important tool for studying PML function in normal and cancerous cells. In normal cells, the PML protein and the PML nuclear bodies play a role in different physiological processes such as: growth control, transcription, DNA repair, DNA replication and RNA transport.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Almacenamiento y estabilidad
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Pondering the promyelocytic leukemia protein (PML) puzzle: possible functions for PML nuclear bodies
Borden KLB
Molecular and Cellular Biology, 22(15), 5259-5269 (2002)
Jason Koshy et al.
Cancer genetics, 205(10), 537-540 (2012-09-18)
Acute promyelocytic leukemia (APL) is a well-defined subtype of acute myeloid leukemia (AML) specifically characterized by the t(15;17)(q22;q12) translocation. The t(15;17) results in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARA) genes. Rare cryptic fusions
Wei Zhou et al.
Cell & bioscience, 10(1), 146-146 (2020-12-24)
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I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts
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