P4020

Protamine sulfate

amorphous powder

• Sinónimos: Salmine, Salmine from salmon
• Número CAS: 53597-25-4
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00132091

Propiedades

• Nombre del producto: Protamine sulfate salt from salmon, Grade X, amorphous powder
• type: Grade X
• Quality Segment: 200
• form: amorphous powder
• impurities: histone, free (Millon test)
• color: white to off-white
• solubility: 6 M HCl: 25 mg/mL

Descripción

General description

Protamine are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. It is found to be localized to the sperm head.

Application

Protamine sulfate salt from salmon has been used:

• in the pretreament of cells to study its effect on respiratory syncytial virus infection
• in lentivirus production
• to derivatize it into a diazeniumdiolate NO donor

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Biochem/physiol Actions

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)