P3369

Protamine sulfate

solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction

• Sinónimos: Salmine sulfate from salmon sperm, Protamine sulfate
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00132091

Propiedades

• Nombre del producto: Protamine sulfate, meets USP testing specifications
• agency: meets USP testing specifications
• Quality Segment: 200
• assay: ≥92% (chromatography), 90.0-110.0% dry basis
• form: solid
• mol wt: M_r 5000-10000
• technique(s): transduction: suitable
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.

Application

Protamine sulfate has been used in the transduction of

• lentiviruses into human embryonic 293T cells
• shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
• lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34⁺ cells

Biochem/physiol Actions

Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.

Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)