P300-05

Porcine Coronary Artery Endothelial: PCAEC (Cryovial)

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: Porcine coronary artery
• Nivel de calidad: 100
• Formulario: solid
• envase: pkg of 500,000 cells
• fabricante / nombre comercial: Cell Applications, Inc
• modo de crecimiento: Adherent
• cariotipo: 2n = 38
• morfología: Endothelial
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cardiovascular diseases
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


Arterial endothelial cells play critical roles in cardiac homeostasis, and insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells provide a useful tool to study artery endothelial function and metabolism.PCAEC from Cell Applications, Inc. have been used to investigate a new mechanism of obesity-associated vascular disease by demonstrating that resistin, an adipocyte-derived cytokine implemented in insulin resistance and diabetes, can cause endothelial dysfunction in coronary arteries through oxidative stress and down-regulation of NO synthase (Kougias, 2005). Conversely, secretoneurin was shown to activate expression of NO synthase and calmodulin, leading to endothelial relaxation (Chan, 2011). PCAEC were also used in adhesion and transmigration experiments in a study investigating anti-inflammatory, cardioprotective effects of a novel adenosine-lidocaine drug formulation (Shi, 2012).

Origen línea celular

Artery

Aplicación

cardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine production

Componentes

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Nota de preparación

• 2nd passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 12 doublings

Rutina de subcultivo

Please refer to the PCAEC Culture Protocol.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable