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P1378

Sigma-Aldrich

Ftaldialdehído

≥97% (HPLC), powder or crystals

Sinónimos:

Dicarboxaldehído o-ftálico, o-Ftalaldehído, Benceno-1,2-dicarboxaldehído, OPA

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About This Item

Fórmula empírica (notación de Hill):
C8H6O2
Número de CAS:
643-79-8
Peso molecular:
134.13
Beilstein/REAXYS Number:
878317
EC Number:
211-402-2
MDL number:
MFCD00003335
UNSPSC Code:
12352201
PubChem Substance ID:
24898191
NACRES:
NA.21
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

200

assay

≥97% (HPLC)

form

powder or crystals

color

white to light yellow

mp

55-58 °C

storage temp.

2-8°C

SMILES string

O=Cc1ccccc1C=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

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Application

El ftaldialdehído se ha utilizado:

  • para detectar Arg a través de espectrofotometría utilizando detección sensible a la fluorescencia de punto final
  • como una corriente reactiva con la muestra para dar lugar a un producto fluorescente destinado a determinar el amoníaco en el agua de mar
  • en el análisis espectrofotométrico del o-ftaldialdehído (prueba OPA) para determinar la actividad proteolítica (PA) de las cepas y los cultivos comerciales

Biochem/physiol Actions

El o-ftaldialdehído (OPA) puede formar derivados fluorescentes mientras reacciona con aminoácidos.
El ftaldialdehído se puede utilizar para la derivatización pre-columna de aminoácidos para la separación mediante cromatografía de líquidos de alto rendimiento (HPLC). También se puede utilizar para mediciones mediante citometría de flujo de los grupos tiol de las proteínas.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

269.6 °F - closed cup

flash_point_c

132 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCM0599
BCCJ0409
BCCF5794
BCCC3481
BCCB5919

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Yun-Bin Han et al.
Applied microbiology and biotechnology, 99(16), 6715-6726 (2015-02-17)
Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
I Molnár-Perl
Journal of chromatography. A, 913(1-2), 283-302 (2001-05-18)
An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have
Bianca de Moraes Fracasso et al.
PloS one, 14(1), e0209964-e0209964 (2019-01-12)
Circulating advanced glycation end products (AGE) and their receptor, RAGE, are increased after a myocardial infarction (MI) episode and seem to be associated with worse prognosis in patients. Despite the increasing importance of these molecules in the course of cardiac
Niklas Krause et al.
Scandinavian journal of work, environment & health, 41(2), 124-139 (2015-01-20)
This study aimed to assess the effects of physically demanding work - measured as energy expenditure (EE) during occupational physical activities (OPA) - on risk of acute myocardial infarction (AMI) among men with and without preexisting ischemic heart disease (IHD).
Yi-Jhen Lai et al.
Talanta, 91, 103-109 (2012-03-01)
This study reports a selective and sensitive method for fluorescent detection of total, protein-bound, free, and free oxidized homocysteine (HCys) using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent, fluorosurfactant-capped gold nanoparticles (FSN-AuNP) as a preconcentrating probe, and o-phthaldialdehyde (OPA) as a
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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is Ortho phthaldehyde HPLC purity of >99% or >97% good enough for use an analytical grade reagent ?

    1 answer

    1. This product has a minimum purity specification of 98%. For analytical purposes, high purity product options are generally preferred. Product P0657, Phthaldialdehyde, offers a minimum purity of 99% and is tested for suitability in fluorometric HPLC.

      Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/sial/p0657

      Helpful?

  4. Has pthaldialdehyde been used to fix tissue and/or cells, and if so, what was the concentration?

    1 answer

    1. The primary use of P1378 is for assaying amines or sulfhydryls in solution. It does not appear to be used as a fixative, although there are reports of its use as a high-level disinfectant. Phthalaldehyde is commonly used as a high-level disinfectant for medical instruments, typically sold under the brand names of Cidex OPA or TD-8. However, in the case of Phthalaldehyde, its use appears to be as a disinfectant rather than as a fixative. The product is tested for appearance, HPLC for purity, color, solubility, and for carbon content. No suitability testing is performed for any specific use.

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