P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Sinónimos: Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• Número de CAS: 77107-46-1
• Número MDL: MFCD00131869
• Código UNSPSC: 12352202
• NACRES: NA.61

Propiedades

• origen biológico: bovine heart
• Nivel de calidad: 200
• Formulario: lyophilized powder
• actividad específica: 2,500-10,000 units/mg protein
• composición: Protein, ≥85% Lowry
• técnicas: calcium flux assay: suitable
• Nº de acceso UniProt: P62157
• temp. de almacenamiento: −20°C
• Información sobre el gen: cow ... CALM3(520277)

Descripción

Aplicación

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Acciones bioquímicas o fisiológicas

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Definición de unidad

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Forma física

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Nota de preparación

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)