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Merck
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N9389

Sigma-Aldrich

Fosfato de 4-nitrofenilo disodium salt hexahydrate

chromogenic, tablet

Sinónimos:

4-Nitrofenil fosfato de disodio, 4-Nitrofenil fosfato de sodio hexahydrate, Fosfato de p-nitrofenilo disodium hexahydrate, pNPP disodium hexahydrate

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About This Item

Fórmula lineal:
O2NC6H4OP(O)(ONa)2 · 6H2O
Número de CAS:
333338-18-4
Peso molecular:
371.14
Beilstein/REAXYS Number:
5324661
EC Number:
224-246-5
MDL number:
MFCD00066288
UNSPSC Code:
12352204
PubChem Substance ID:
24897898
NACRES:
NA.83

product name

Fosfato de 4-nitrofenilo disodium salt hexahydrate, tablet

description

5 mg substrate per tablet

form

tablet

fluorescence

λex 310 nm (pH 7.1)
λex 399 nm (Reaction Product)

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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General description

Sustrato cromógeno para la determinación de fosfatasas ácidas y alcalinas. La reacción da un producto final soluble que es de color amarillo. La reacción puede detenerse con NaOH 3 N y leerse a 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
  • enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)
  • enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA
  • alkaline phosphatase assay

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Xiaohong Wang et al.
Biochemical pharmacology, 102, 97-106 (2016-01-06)
Inorganic polyphosphate (polyP) is a physiological polymer composed of tens to hundreds of phosphate units linked together via phosphoanhydride bonds. Here we compared the biological activity of polyP (chain length of 40 phosphate units), complexed with Gd(3+) (polyP·Gd), with the
J Eckl-Dorna et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 42(9), 1347-1355 (2012-08-29)
The production of allergen-specific IgE antibodies is a hallmark of IgE-mediated allergy but the contribution of blood cells to allergen-specific IgE production in allergic patients has not been studied in detail. Aim of this study was the characterization of IgE-producing
Marianne K Schesny et al.
Biomaterials, 35(25), 7180-7187 (2014-05-31)
Chemokine-induced stem cell recruitment is a promising strategy for post myocardial infarction treatment. Injection of stromal cell-derived factor 1 (SDF1) has been shown to attract bone marrow-derived progenitor cells (BMPCs) from the blood that have the potential to differentiate into
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
Rasmus Iversen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(48), 17146-17151 (2014-11-19)
The multifunctional enzyme transglutaminase 2 (TG2) is the target of autoantibodies in the gluten-sensitive enteropathy celiac disease. In addition, the enzyme is responsible for deamidation of gluten peptides, which are subsequently targeted by T cells. To understand the regulation of
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