MAK318
α−Mannosidase Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Productos recomendados
detection method
colorimetric
relevant disease(s)
neurological disorders
storage temp.
2-8°C
Categorías relacionadas
General description
α-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.
Principle
The α-Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining α-Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.
One unit of AMA is the amount of enzyme that will convert 1.0 μmole of 4-Nitrophenyl-α-D-mannopyranoside to 4-Nitrophenol and α-D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.
One unit of AMA is the amount of enzyme that will convert 1.0 μmole of 4-Nitrophenyl-α-D-mannopyranoside to 4-Nitrophenol and α-D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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