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M4892

McCoy′s 5A Medium

Name: McCoy′s 5A Medium
Brand: SIGMA

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

5A basal medium

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UNSPSC Code:

12352207

NACRES:

NA.71

MDL number:

MFCD00217560

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Propiedades

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO₃: no

shipped in

ambient

storage temp.

2-8°C

Descripción

General description

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used for culturing:

human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
human colorectal HCT116 cells to study miR-224 over-expression in cancer
osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Formulated to contain 11.9 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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Este artículo	M8403	M2154	M9309
SAFC M4892 McCoy′s 5A Medium	Sigma-Aldrich M8403 McCoy′s 5A Medium	Sigma-Aldrich M2154 Medium 199	Sigma-Aldrich M9309 McCoy′s 5A Medium
description Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
form powder	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components phenol red: 0.011 g/L L-glutamine: 0.21915 g/L glucose: 3.0 g/L (Dextro) NaHCO₃: no	components L-glutamine: no NaHCO₃: 2.2 g/L glucose: 3.0 g/L phenol red: 0.011 g/L	components sodium pyruvate: no L-glutamine: no NaHCO₃: yes HEPES: no phenol red: yes Earle’s salts (5% CO₂): yes	components NaHCO₃: 2.2 g/L L-glutamine: 0.21 g/L phenol red: 0.011 g/L glucose: 3.0 g/L (Dextro)
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 500	Quality Level 400	Quality Level 400	Quality Level 300

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