A typical LPS molecule has lipid A (a glucosamine-based phospholipid), a relatively short core oligosaccharide, and a distal polysaccharide (O-antigen). That means this product contains lipid A but is not comprised entirely of lipid A. The molecular weight is not determined.
L9641
Lipopolysaccharides (rough strains) from Escherichia coli EH100 (Ra mutant)
Sinónimos:
LPS
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Permítanos ayudarlebiological source
Escherichia coli (EH100 Ra mutant)
Quality Segment
form
lyophilized powder
impurities
<3% Protein (Lowry)
color
white to yellow
solubility
water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
General description
This product is phenol:chloroform:petroleum ether extracted from E. coli EH 100 (Ra mutant). The source strain is from a private collection.
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
Prepared by phenol-chloroform-petroleum ether extraction.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Este artículo | |||
|---|---|---|---|
| biological source Escherichia coli (EH100 Ra mutant) | biological source Escherichia coli (F583 Rd mutant) | biological source Escherichia coli (O111:B4) | biological source Escherichia coli (J5 Rc mutant) |
| solubility water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow | solubility water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow | solubility water: soluble | solubility water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities <3% Protein (Lowry) | impurities <3% Protein (Lowry) | impurities - | impurities <3% Protein (Lowry) |
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is this item a Lipid A? What is the molecular weight?
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