The molecular weight of Concanavalin A is 102 kDa. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/169/422/l7647pis.pdf
L7647
Concanavalin A from Canavalia ensiformis (Jack bean)
Type VI, lyophilized powder
Sinónimos:
Con A
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Form:
lyophilized powder
Biological source:
Canavalia ensiformis
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Servicio técnico
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Permítanos ayudarlebiological source
Canavalia ensiformis
Quality Segment
type
Type VI
form
lyophilized powder
potency
≤20 μg per mL agglutination activity
impurities
Carbohydrate, essentially free, salt, essentially free
solubility
water: 1 % (w/v)
storage temp.
−20°C
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used for developing zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been also used in the preparation of protein loaded microgels to prevent enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Preparation Note
Highly purified by specific affinity chromatography.
Analysis Note
Agglutination activity: Determined from serial dilutions of a 1 mg/mL solution (this activity is the lowest conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.
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Este artículo | |||
|---|---|---|---|
| biological source Canavalia ensiformis | biological source Canavalia ensiformis | biological source - | biological source Canavalia ensiformis (jack bean) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| impurities Carbohydrate, essentially free, salt, essentially free | impurities Carbohydrate, essentially free | impurities Carbohydrate, essentially free, salt, essentially free | impurities carbohydrate, essentially free |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| potency ≤20 μg per mL agglutination activity | potency - | potency <64 μg per mL agglutination activity | potency - |
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signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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