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L5667

LB Agar Ampicillin-100, Plates

pre-poured LB agar plates with 100 μg/ml ampicillin

Sinónimos:

ampicillin agar plates

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UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli, selective for coliforms
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grade

Molecular Biology

Quality Level

sterility

sterile; autoclaved

form

plates (10 cm), plates (10cm, 20-25ml each)

availability

available only in USA

capacity

20-25 mL

storage temp.

2-8°C

suitability

selective for Escherichia coli, selective for coliforms

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

LB Agar Ampicillin-100, Plates has been used to plate and incubate the mixture of thawed bacteria and plasmid solution for vimentin expression. It has also been used in the generation of the Trypanosoma brucei RNAi library.

Features and Benefits

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Other Notes

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
100μg/ml ampicillin
The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

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Este artículo
L0543L3147L2542
sterility

sterile; autoclaved

sterility

sterile; autoclaved

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

suitability

selective for Escherichia coli, selective for coliforms

suitability

selective for Escherichia coli, selective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

form

plates (10 cm), plates (10cm, 20-25ml each)

form

plates (10 cm), plates (10cm, 20-25ml each)

form

powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

capacity

20-25 mL

capacity

20-25 mL

capacity

-

capacity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400


Clase de almacenamiento

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes
Glover L, et al.
Nature Protocols, 10(1), 106-106 (2015)
Linfeng Xu et al.
Nature communications, 12(1), 6803-6803 (2021-11-25)
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation.
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science Advances, 4(6) (2018)



Número de artículo de comercio global

SKUGTIN
L5667-10EA04061833958346

Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. Why do the LB-agar plates with antibiotics have such a short expiration date?

    1 answer
    1. The antibiotics in the LB-agar plates are not stable.  The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate.  Because of this, we recommend the plates are used as soon as possible once they are received.  For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).

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