L5418

Lipopolysaccharides from Escherichia coli O55:B5

Ready Made solution, 1 mg/mL

• Sinónimos: LPS
• Número de CAS: 93572-42-0
• Número MDL: MFCD00164401
• Código UNSPSC: 12352201
• NACRES: NA.25

Propiedades

• origen biológico: Escherichia coli (O55:B5)
• Nivel de calidad: 200
• Formulario: aqueous solution
• concentración: 1 mg/mL
• color: white to faint yellow
• solubilidad: water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O55:B5. The source strain is CDC 1644-70. This LPS serotype has been used in studies of septic shock and to induce myocarditis.

Aplicación

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Acciones bioquímicas o fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparación

The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.

Otras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)