L2005

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥200 units/mg protein

• Sinónimos: Donor:hydrogen peroxide oxidoreductase, Peroxidase
• Número CAS: 9003-99-0
• NACRES: NA.54
• UNSPSC Code: 12352204
• EC Number: 232-668-6
• MDL number: MFCD00131484
• Número CE: 1.11.1.7 (BRENDA, IUBMB)
• eCl@ss: 32160410
• Specific activity: ≥200 units/mg protein
• Biological source: bovine milk

Propiedades

• biological source: bovine milk
• Quality Segment: 200
• form: lyophilized powder (essentially salt-free)
• specific activity: ≥200 units/mg protein
• absorbance ratio: A_{412/280 nm} 0.7-0.9
• UniProt accession no.: P80025
• storage temp.: −20°C
• SMILES string: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• Gene Information: cow ... LPO(280844)

Descripción

General description

Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.

Application

Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H₂O₂ in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.

Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.

Analysis Note

Protein determined by Lowry method.

Other Notes

One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)