K4002

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

• Sinónimos: Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
• NACRES: NA.75
• UNSPSC Code: 12161702

Propiedades

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• pH: 6.1-6.7
• solubility: water: 9.5 g/L, clear
• storage temp.: 2-8°C

Descripción

General description

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Application

Krebs-Ringer Bicarbonate Buffer has been used:

• as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
• in the pre-perifusion for islet viability and functionality studies
• in pituitary bioassay

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.

Supplement with 1.26 g/L sodium bicarbonate.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable