I5907

Pyrophosphatase, Inorganic from Escherichia coli

recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein

• Sinónimos: Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
• Número de CAS: 9024-82-2
• Comisión internacional de enzimas: 3.6.1.1 (BRENDA, IUBMB)
• EC Number: 232-784-7
• MDL number: MFCD00131379
• UNSPSC Code: 12352204
• NACRES: NA.32

Propiedades

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: ≥90%
• form: lyophilized powder
• specific activity: ≥800 units/mg protein
• mol wt: hexamer subunit mol wt 20 kDa
• storage temp.: −20°C

Descripción

General description

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity.[1] Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.[2]

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.[3]

Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins.[4] It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.[5]

Biochem/physiol Actions

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria[6] The active site residues are crucial for binding to magnesium.[6]

Other Notes

A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg²⁺ dependent. It is a relatively thermostable protein.

Unit Definition

One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

Physical form

Lyophilized powder in Tris-buffered salts containing protease inhibitors

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)