H7787
HIS-Select® Spin Columns
Sinónimos:
His-Tag Spin Columns
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NACRES:
NA.56
UNSPSC Code:
41106500
Pore size:
100 nm pore size
Packaging:
pkg of 10 ea, pkg of 50 ea
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleshelf life
2 yr at 2‑8 °C
Quality Level
packaging
pkg of 10 ea, pkg of 50 ea
pore size
100 nm pore size
capacity
>0.5 mg/column
storage temp.
2-8°C
General description
Kit contains 3 collection tubes for every spin column.
Application
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.
Features and Benefits
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The Journal of biological chemistry, 285(38), 29147-29155 (2010-07-24)
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Anne Durand et al.
The Journal of biological chemistry, 281(40), 29558-29567 (2006-07-26)
In Escherichia coli, the ammonia channel AmtB and the P(II) signal transduction protein GlnK constitute an ammonium sensory system that effectively couples the intracellular nitrogen regulation system to external changes in ammonium availability. Binding of GlnK to AmtB apparently inactivates
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| H7787-10EA | 04061835573271 |
| H7787-50EA | 04061833804759 |