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Merck

H6136

Hanks′ Balanced Salts

Without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Hanks′ Balanced Salt Solution

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UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Servicio técnico
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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).

Application

Hanks′ Balanced Salt has been used:

  • in the preparation of stock solution for culturing mice primary hepatocytes
  • to measure the mineralized nodule formation in human osteoblast cultures
  • in the preparation of embryo buffer to collect zebrafish eggs

Preparation Note

Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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