GUSS
β-Glucuronidase Reporter Gene Staining Kit
Sinónimos:
β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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uso
kit sufficient for 100 histochemical assays
Nivel de calidad
técnicas
microbe id | staining: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.
Aplicación
The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Características y beneficios
- Histochemical staining of plant tissues expressing the E. coli GUS enzyme
- Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
- GUS does not interfere with plant cell function or viability
Otras notas
The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.
Solo componentes del kit
Referencia del producto
Descripción
- 2× Fixation Buffer 25 mL
- 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
- Reagent A for gus staining kit 50 mL
- Reagent B for gus staining kit 200 μL
- Reagent C for gus staining kit 200 μL
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3
Código de clase de almacenamiento
11 - Combustible Solids
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We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We
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