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GL0010

Sigma-Aldrich

Golgi Isolation Kit

sufficient for 50 g (tissue)

Sinónimos:

Golgi Kit, Isolation Kit for Golgi

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

uso

sufficient for 50 g (tissue)

Nivel de calidad

200

técnicas

fractionation: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

Aplicación

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

Nota de análisis

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDS

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

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Certificados de análisis (COA)

Lot/Batch Number
0000195672
0000195672
11180745
029M4147V
058M4161V

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Julien Villeneuve et al.
The Journal of cell biology, 217(2), 649-665 (2017-12-08)
An appreciation of the functional properties of the cytoplasmic fatty acid binding protein 4 (FABP4) has advanced with the recent demonstration that an extracellular form secreted by adipocytes regulates a wide range of physiological functions. Little, however, is known about
A Surroca et al.
The Journal of membrane biology, 177(3), 243-249 (2000-10-03)
We investigated the direct effect of inositol 1,4,5-trisphosphate (IP(3)) and ryanodine receptor agonists on Ca(2+) release from vesicles of a rat liver Golgi apparatus (GA) enriched fraction, which were actively loaded with (45)Ca(2+). Results in GA were compared with those
E Prchla et al.
The Journal of cell biology, 131(1), 111-123 (1995-10-01)
Endosomal penetration by nonenveloped viruses might be accomplished by either local breakdown of the endosomal membrane (e.g., adenovirus) or formation of a membrane-spanning pore by capsid proteins. Uncoating of the nonenveloped virus human rhinovirus serotype 2 (HRV2) has been shown
E R Sjoberg et al.
The Journal of biological chemistry, 268(14), 10185-10196 (1993-05-15)
The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized. However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure. Here, we have studied the spatial and temporal interrelationships among the
Martin J Lear et al.
Journal of natural products, 72(11), 1980-1987 (2009-10-22)
(+/-)-Laetirobin (1) was isolated as a cytostatic lead from Laetiporus sulphureus growing parasitically on the black locust tree, Robinia pseudoacacia, by virtue of a reverse-immunoaffinity system. Using an LC/MS procedure, milligram quantities of (+/-)-laetirobin (1) were obtained, and the structure
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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