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GE28-9909-46

Sigma-Aldrich

Superdex® 200 Increase 3.2/300

column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

Sinónimos:

Superdex 200 Increase

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

glass column
titanium frit

product line

Superdex®

mol wt

10000-600000 Da (globular proteins)

packaging

1 ea of

parameter

<0.15 ml/min flow rate
3.0 Mpa (652 psi)
4-50 mL sample volume

bed volume

~2.4 mL

column L × I.D.

30 cm × 3.2 mm

matrix

agarose-dextran composite
polymeric

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superdex 200 is a column for high resolution gel filtration in micro scale (μg) preparative purification as well as characterization and analysis of small sample volumes (4-50 μL) of proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Publishing

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification as well as characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Suitability

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
for both preparative and analytical purposes

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Ver todo

Contenido relacionado

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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