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GE28-9365-50

GSTPrep™ Fast Flow 16/10

Cytiva 28-9365-50

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About This Item

Código UNSPSC:
13111000
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

envase

pkg of 20 mL

fabricante / nombre comercial

Cytiva 28-9365-50

Parámetros

<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

lecho tamaño

16 mm × 100 mm

lecho volumen

20 mL

columna D.I.

16 mm

Matriz

highly cross-linked 4% agarose

diámetro promedio

90 μm

cleaning

3-12

Intervalo de trabajo

3-12

capacidad

>10 mg/mL binding capacity

Descripción general

GSTPrep™ FF 16/10 is prepacked, ready-to-use column containing Glutathione Sepharose™ 4 Fast FLow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Aplicación

Glutathione Sepharose™ 4 Fast FLow is for scale-up purification of recombinant GST-tagged Proteins, other glutathione S-transferases, and glutathione-dependent Proteins. Mild elution conditions preserve Protein antigenicity and function.

Características y beneficios

  • Fast and easy one-step, scale-up purification of GST-tagged Proteins.
  • Binds approx. 200 mg recombinant GST/column
  • Mild elution conditions preserving Protein antigenicity and function.
  • Simple operation with a pump or chromatography system, such as ÄKTA® design.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

GSTPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Protocolos

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

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