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GE17-0920-07

IMAC Sepharose High Performance

Cytiva 17-0920-07, pack of 100 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

envase

pack of 100 mL

fabricante / nombre comercial

Cytiva 17-0920-07

Matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

Intervalo de trabajo

3-12

capacidad

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Descripción general

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Aplicación

Conveniently charge with your metal of choice. IMAC Sepharose FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

Características y beneficios

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
  • Conveniently charge with your metal of choice
  • 34 μm bead size for high performance purification
  • High binding capacity

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


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Certificados de análisis (COA)

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Artículos

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

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Protocolos

Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

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