GE17-0034-02
Sephadex® G-25 Coarse
Cytiva 17-0034-02, pack of 500 g
About This Item
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envase
pack of 500 g
fabricante / nombre comercial
Cytiva 17-0034-02
condiciones de almacenamiento
dry media
Matriz
cross-linked dextran
tamaño de partícula
>100 μm (dry)
cleaning in place
2-13
Intervalo de trabajo
2-13
idoneidad
suitable for bioprocess medium
Descripción general
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).
Sephadex® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Características y beneficios
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Excellent recovery and minimum sample dilution
- Available in prepacked HiPrep™ Desalting and HiTrap® Desalting columns for fast and convenient desalting
- BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications
Almacenamiento y estabilidad
Nota de análisis
Información legal
Código de clase de almacenamiento
13 - Non Combustible Solids
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Certificados de análisis (COA)
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Artículos
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocolos
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to prepare samples for purification with affinity chromatography.
Contenido relacionado
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
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