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GALS

Sigma-Aldrich

β-Galactosidase Reporter Gene Staining Kit

Sinónimos:

β-galactosidase, LacZ

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.32

usage

 kit sufficient for 100 tests (using a 3.5 cm dish)

Quality Level

200

technique(s)

microbe id | staining: suitable

shipped in

dry ice

storage temp.

−20°C

General description

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Application

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
  • sagittal brain sections in histoenzymology
  • pancreas or colon sections in β-galactosidase reporter gene assay
  • mice E10.5 -11.5 embryo and embryo sections

Other Notes

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

related product

Referencia del producto
Descripción
Precios

94433

β-Galactosidase stain

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

supp_hazards

EUH032

Storage Class

3 - Flammable liquids

flash_point_f

132.8 °F

flash_point_c

56 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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D Long et al.
Journal of virology, 68(9), 5890-5898 (1994-09-01)
Human immunodeficiency virus type 1 (HIV-1) infects some cell types which lack CD4, demonstrating that one or more alternative viral receptors exist. One such receptor is galactosylceramide (GalCer), a glycosphingolipid distributed widely in the nervous system and in colonic epithelial
GPR120 (FFAR4) is preferentially expressed in pancreatic delta cells and regulates somatostatin secretion from murine islets of Langerhans
Stone VM, et al.
Diabetologia, 57(6), 1182-1191 (2014)
Maria Boichuck et al.
Aging, 11(9), 2889-2897 (2019-05-16)
Here, we reported for the first time an increased expression of c-Met protein in primary cultures of human dermal and pulmonary fibroblasts of late passages. This suggests that c-Met could serve as an early marker of cellular senescence (CS). The
Wendy Phillips et al.
Methods in molecular biology (Clifton, N.J.), 549, 137-155 (2009-04-21)
Animal models of neurodegenerative disease are excellent tools for studying pathogenesis and therapies including cellular transplantation. In this chapter, we describe different models of Huntington's disease and Parkinson's disease, stereotactic surgery (used in creation of lesion models and transplantation) and
beta-Galactosidase assay
Smale ST
Cold Spring Harbor Protocols, 2010, pdb-prot5423 (2010)
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