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Merck

G7402

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Gold antibody produced in goat

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

gold conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

aqueous glycerol suspension

técnicas

dot blot: suitable
western blot: suitable

tamaño de partícula

10 nm (colloidal gold)

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-rabbit IgG (whole molecule)-gold antibody can be used as secondary antibody (diluted 1:50 in 0.01 M PBS containing 1% BSA and 5% NGS) in EM immunolabeling of GABA and glutamate by postembedding procedure. It can also be used in a modified dot blot assay to determine binding activity. Goat anti-rabbit IgG (whole molecule)-gold antibody reacts specifically with all goat immunoglobulins.
Immunoglobulins (Igs) are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs can be useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Gold antibody is specific for rabbit IgG.

Inmunógeno

Rabbit IgG purified from normal rabbit serum.

Aplicación

Anti-rabbit IgG (whole molecule)-gold antibody (diluted 1: 15) can be used in immunoelectron microscopy. It can also be used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)
Immunohistochemistry (1 paper)

Forma física

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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G Kamp et al.
Reproduction (Cambridge, England), 133(1), 29-40 (2007-01-25)
Glycolysis is crucial for sperm functions (motility and fertilization), but how this pathway is regulated in spermatozoa is not clear. This prompted to study the location and the regulatory properties of 6-phosphofructokinase (PFK, EC 2.7.1.11), the most important element for
S Sinnarajah et al.
FEBS letters, 426(3), 377-380 (1998-05-26)
The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence
Andrea González et al.
The American journal of tropical medicine and hygiene, 92(5), 887-897 (2015-03-12)
Trypanosoma cruzi calreticulin (TcCRT), a 47-kDa chaperone, translocates from the endoplasmic reticulum to the area of flagellum emergence. There, it binds to complement components C1 and mannan-binding lectin (MBL), thus acting as a main virulence factor, and inhibits the classical
R Fabian-Fine et al.
The Journal of comparative neurology, 420(2), 195-210 (2001-02-07)
The mechanosensory neurons of arachnids receive diverse synaptic inputs in the periphery. The function of most of these synapses, however, is unknown. We have carried out detailed electron microscopic investigations of the peripheral synapses at sensory neurons in the compound
Ignaz Wessler et al.
PloS one, 11(6), e0156886-e0156886 (2016-06-11)
The worldwide use of neonicotinoid pesticides has caused concern on account of their involvement in the decline of bee populations, which are key pollinators in most ecosystems. Here we describe a role of non-neuronal acetylcholine (ACh) for breeding of Apis

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