F4042

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Anti-ddddk, Anti-dykddddk
• UNSPSC Code: 12352203
• NACRES: NA.32
• MDL number: MFCD00282246
• Conjugate: unconjugated
• Clone: M5, monoclonal
• Application: WB CL
• Citations: 186

Propiedades

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M5, monoclonal
• form: buffered aqueous solution
• purified by: using Protein A
• species reactivity: all
• concentration: 2-5 mg/mL
• technique(s): western blot (chemiluminescent): 10 μg/mL
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Descripción

General description

Monoclonal ANTI-FLAG^® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca²⁺-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Preparation Note

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Other Notes

Antibody is not calcium dependent.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable