F1177
Fluorescein hyaluronic acid
>95%, powder
Sinónimos:
Hyaluronic Acid Fluorescein Conjugate
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About This Item
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Quality Level
assay
>95%
form
powder
mol wt
~800,000 Da
color
orange
solubility
H2O: soluble 1 mg/mL
storage temp.
2-8°C
Application
Biochem/physiol Actions
Hyaluronan macromolecules (HAs) coupled to fluorescein amine is exploited to screen the hyaluronidase activity using fluorescence correlation spectroscopy (FCS) by measuring the quenching of fluorescein-labeled HA fragments.[4] It binds on cell surface of malignant mesothelioma derived from human tumors.[5]
Used to bind on cell surface of malignant mesothelioma derived from human tumors.
Other Notes
From cock′s comb or by fermentation.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Los clientes también vieron
Hyaluronan receptors are expressed on human malignant mesothelioma cells but not on normal mesothelial cells
Asplund T and Heldin P
Cancer research, 54(16), 4516-4523 (1994)
Rafal Fudala et al.
Journal of photochemistry and photobiology. B, Biology, 106, 69-73 (2011-11-16)
In this report we propose a lifetime-based sensing (LBS) for the detection of hyaluronidase (HA-ase). First, we heavily label hyaluronan macromolecules (HAs) with fluorescein amine. The fluorescein labeled HA (HA-Fl) has a weak fluorescence and short fluorescence lifetime due to
Human macrophage adhesion on polysaccharide patterned surfaces
Tsai IY, et al.
Soft Matter, 7(7), 3599-3606 (2011)
Rheology and confocal reflectance microscopy as probes of mechanical properties and structure during collagen and collagen/hyaluronan self-assembly
Yang Yl and Kaufman LJ
Biophysical Journal, 96(4), 1566-1585 (2009)
T Asplund et al.
Cancer research, 54(16), 4516-4523 (1994-08-15)
Hyaluronan-binding sites were demonstrated on the cell surface of three malignant mesothelioma cell lines derived from human tumors using either [3H]hyaluronan or fluorescein-tagged hyaluronan. No hyaluronan-binding activity was observed on normal human mesothelial cells. The absence of hyaluronan receptors on
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